⚡ Unit X Overview: Modern Experimental Techniques in Cell Biology
Unit X investigates cutting-edge experimental methodologies in cytology: light, confocal, super-resolution and electron microscopy; mammalian cell culture, transfection, and CRISPR-Cas9 genome editing; and subcellular fractionation, flow cytometry, and proteomics.
Unit Curriculum & Detailed Lecture Notes
Select a subtopic below to explore comprehensive university-level lecture notes, high-resolution vector diagrams, and mechanistic breakdowns:
Microscopy: Light, Confocal & EM
Abbe diffraction limits, Phase-contrast, DIC, Laser Scanning Confocal pinholes, STED super-resolution, and TEM/SEM electron optics.
Cell Culture & Genetic Engineering
Primary vs continuous cell lines, Hayflick limit, BSL biosafety cabinets, and CRISPR-Cas9 target recognition and DSB repair mechanisms.
Molecular Biology Methods in Cytology
Differential and sucrose density gradient ultracentrifugation, FACS flow cytometry, Western blotting, and co-immunoprecipitation.
