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Plant Biotechnology

I. Introduction to Plant Biotechnology

  • Historical overview of plant biotechnology
  • Overview of plant cell culture
  • Overview of genetic engineering techniques

II. Plant Tissue Culture

  • Plant tissue culture techniques
    1. Callus culture
    2. Micropropagation
    3. Organogenesis
    4. Somatic embryogenesis
    5. Protoplast culture
    6. Anther culture
    7. Embryo rescue
  • Plant regeneration

III. Genetic Engineering of Plants

  • Gene transfer methods
    1. Agrobacterium-mediated transformation
    2. Particle bombardment / Biolistic transformation
    3. Electroporation
    4. Liposome-mediated transformation
    5. Chemical transformation / PEG-mediated transformation
    6. Tissue culture
    7. Gene editing
    8. RNA interference (RNAi)
    9. Mutagenesis
  • Transgenic plant production

IV. Applications of Plant Biotechnology

  • Molecular markers and genotyping
  • Genetic modification for improved crop traits
  • Plant biotechnology for stress tolerance and disease resistance
  • Plant biotechnology for the production of pharmaceuticals and biofuels

V. Regulatory and Ethical Issues in Plant Biotechnology

  • Regulations governing the use of genetically modified crops
  • Ethical considerations in the use of plant biotechnology
  • Public perception and communication of plant biotechnology
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